Hotspot

rpl33-rps18

LSC · 70236-70427 · 192 bp

Back to Phellodendron

Pi 0.0469
MarkerSeek score 79.50
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
192
variable_sites
9
pi
0.0469
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 ACATACACTTCACGGGGAGA TCAATTCGATCCCCCGATTG 50.0 50.0 58.1 57.7 309-350 bp 1.0 79.6
rpl33-rps18_p2 ACATACACTTCACGGGGAGA ATTCGATCCCCCGATTGGAT 50.0 50.0 58.1 58.6 306-347 bp 1.0 76.6
rpl33-rps18_p3 ACATACACTTCACGGGGAGA CAATTCGATCCCCCGATTGG 50.0 55.0 58.1 58.8 308-349 bp 1.0 75.6
rpl33-rps18_p4 ACATACACTTCACGGGGAGA AATTCGATCCCCCGATTGGA 50.0 50.0 58.1 58.9 307-348 bp 1.0 74.8
rpl33-rps18_p5 ACATACACTTCACGGGGAGA GCAAACGCCTACGAAAAGAT 50.0 45.0 58.1 57.1 281-322 bp 1.0 74.7