Hotspot

psaJ-rpl33

LSC · 69537-70034 · 498 bp

Back to Phellodendron

Pi 0

MarkerSeek score 37.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	GGACATCTTTACCCTTGGCC	50.0	55.0	58.0	58.2	640 bp	1.0	77.8
psaJ-rpl33_p2	TCGGGTCTTTAGCAGGGTTA	GGACATCTTTACCCTTGGCC	50.0	55.0	58.1	58.2	600 bp	1.0	77.6
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	ACACCGTTTCGAACACAACT	50.0	45.0	58.0	58.3	686 bp	1.0	77.5
psaJ-rpl33_p4	TCGGGTCTTTAGCAGGGTTA	ACACCGTTTCGAACACAACT	50.0	45.0	58.1	58.3	646 bp	1.0	77.4
psaJ-rpl33_p5	TGGCACCGGTACTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	627 bp	1.0	76.3