Hotspot

atpF-atpH

LSC · 13881-14278 · 398 bp

Back to Phellodendron

Pi 0

MarkerSeek score 54.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	579-580 bp	1.0	73.7
atpF-atpH_p2	GCCAGTGAACCAAAGAAACG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	553-554 bp	1.0	73.7
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	583-584 bp	1.0	73.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	580-581 bp	1.0	73.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	581-582 bp	1.0	73.6