Hotspot

trnR-UCU-atpA

LSC · 10724-10936 · 213 bp

Back to Phellodendron

Pi 0.0769

MarkerSeek score 86.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 16
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CTGAAAGGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.2	58.1	366-367 bp	1.0	82.4
trnR-UCU-atpA_p2	CTGAAAGGCGTCCATTGTCT	GAAGCCCTTTTGCAAGAAGC	50.0	50.0	58.2	58.2	357-358 bp	1.0	81.5
trnR-UCU-atpA_p3	CTGAAAGGCGTCCATTGTCT	TCAGGAACAAATGGAACGCT	50.0	45.0	58.2	57.7	334-335 bp	1.0	81.4
trnR-UCU-atpA_p4	CTGAAAGGCGTCCATTGTCT	AGCCCTTTTGCAAGAAGCTA	50.0	45.0	58.2	57.7	355-356 bp	1.0	80.9
trnR-UCU-atpA_p5	ACTGAAAGGCGTCCATTGTC	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.5	58.1	367-368 bp	1.0	80.1