Hotspot

matK-trnK-UUU

LSC · 3665-4389 · 725 bp

Back to Phellodendron

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCCGAAAAAGAAGTGGG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	828 bp	1.0	76.5
matK-trnK-UUU_p2	ACTCCCGAAAAAGAAGTGGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	827 bp	1.0	76.5
matK-trnK-UUU_p3	CTCCCGAAAAAGAAGTGGGT	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	827 bp	1.0	76.5
matK-trnK-UUU_p4	CTCCCGAAAAAGAAGTGGGT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	826 bp	1.0	76.5
matK-trnK-UUU_p5	CTCCCGAAAAAGAAGTGGGT	TTGGGTTGCTAACTCAACGG	50.0	50.0	57.7	58.4	842 bp	1.0	74.6