Hotspot

ycf1-chlN

Genome · 123952-124373 · 422 bp

Back to Phegopteris

Pi 0.133
MarkerSeek score 79.50
Primer available yes
Label ycf1-chlN

Feature Metadata

label_name
ycf1-chlN
feature_type
igs
region
Genome
length_bp
422
variable_sites
52
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-chlN_p1 CCGGAGCTCTACCCAGATAA AGTTACTCGTCCATTGCGAC 55.0 50.0 58.0 58.0 1148-1171 bp 1.0 88.3
ycf1-chlN_p2 CCGGAGCTCTACCCAGATAA GTCCATTGCGACGTAGAAGT 55.0 50.0 58.0 58.0 1140-1163 bp 1.0 88.3
ycf1-chlN_p3 CCGGAGCTCTACCCAGATAA CCATTGCGACGTAGAAGTGA 55.0 50.0 58.0 58.0 1138-1161 bp 1.0 88.3
ycf1-chlN_p4 CCGGAGCTCTACCCAGATAA TCCATTGCGACGTAGAAGTG 55.0 50.0 58.0 58.0 1139-1162 bp 1.0 88.3
ycf1-chlN_p5 CCGGAGCTCTACCCAGATAA CCTCGCAGAAATACACCGAA 55.0 50.0 58.0 58.0 1776-1799 bp 1.0 86.5