Hotspot

trnP-GGG-trnL-UAG

Genome · 108928-109222 · 295 bp

Back to Phegopteris

Pi 0.136
MarkerSeek score 81.60
Primer available yes
Label trnP-GGG-trnL-UAG

Feature Metadata

label_name
trnP-GGG-trnL-UAG
feature_type
igs
region
Genome
length_bp
295
variable_sites
36
pi
0.136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-trnL-UAG_p1 GATTCGAACCTGTGACCTCC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 394-410 bp 1.0 90.9
trnP-GGG-trnL-UAG_p2 GATTCGAACCTGTGACCTCC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 392-408 bp 1.0 90.9
trnP-GGG-trnL-UAG_p3 GGATTCGAACCTGTGACCTC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 395-411 bp 1.0 90.9
trnP-GGG-trnL-UAG_p4 GGATTCGAACCTGTGACCTC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 393-409 bp 1.0 90.9
trnP-GGG-trnL-UAG_p5 GATTCGAACCTGTGACCTCC TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.0 57.9 381-397 bp 1.0 90.3