Hotspot

petG-trnW-CCA

Genome · 62005-62283 · 279 bp

Back to Phegopteris

Pi 0.0919

MarkerSeek score 73.70

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: Genome
length_bp: 279
variable_sites: 25
pi: 0.0919
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GCCGTTGTCCAAACCAAAAA	GGCCGTGGAGAGGTATATCT	45.0	55.0	58.0	58.1	1679 bp	0.5	59.3
petG-trnW-CCA_p2	ACGCAGAAATCCACTTTGGT	GGCCGTGGAGAGGTATATCT	45.0	55.0	58.0	58.1	1169 bp	0.5	59.3
petG-trnW-CCA_p3	TTGGTTGACGCAGAAATCCA	GGCCGTGGAGAGGTATATCT	45.0	55.0	58.0	58.1	1176 bp	0.5	59.2
petG-trnW-CCA_p4	GCCGTTGTCCAAACCAAAAA	CAAAACCCGATGTCGTAGGT	45.0	50.0	58.0	57.9	1005 bp	0.5	59.2
petG-trnW-CCA_p5	ATTCCGTGACTTGCTGTTGT	GGCCGTGGAGAGGTATATCT	45.0	55.0	58.0	58.1	1283 bp	0.5	59.1