Hotspot

psbE-petL

Genome · 60856-61626 · 771 bp

Back to Phegopteris

Pi 0.0844

MarkerSeek score 81.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 771
variable_sites: 61
pi: 0.0844
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACTTCTTGTCGACTCCCTGA	ACCAAAGTGGATTTCTGCGT	50.0	45.0	58.0	58.0	1180 bp	0.5	59.8
psbE-petL_p2	TTTGTCATCTGCGCTACCTC	ACCAAAGTGGATTTCTGCGT	50.0	45.0	58.0	58.0	1635 bp	0.5	59.8
psbE-petL_p3	ACTTCTTGTCGACTCCCTGA	TGGATTTCTGCGTCAACCAA	50.0	45.0	58.0	58.0	1173 bp	0.5	59.8
psbE-petL_p4	TTTGTCATCTGCGCTACCTC	TGGATTTCTGCGTCAACCAA	50.0	45.0	58.0	58.0	1628 bp	0.5	59.7
psbE-petL_p5	ACTTCTTGTCGACTCCCTGA	ACAACAGCAAGTCACGGAAT	50.0	45.0	58.0	58.0	1066 bp	0.5	59.7