Hotspot

rps4-trnL-UAA

Genome · 44195-44831 · 637 bp

Back to Phegopteris

Pi 0.0795

MarkerSeek score 81.90

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 637
variable_sites: 49
pi: 0.0795
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	TCACTTTCAAACGAGGTCCTC	CTGCCACTTGATACGTCTCC	47.6	55.0	57.9	58.1	746-747 bp	1.0	78.1
rps4-trnL-UAA_p2	TCACTTTCAAACGAGGTCCTC	GCGTCTACCATTTCGCCATA	47.6	50.0	57.9	58.1	696-697 bp	1.0	78.1
rps4-trnL-UAA_p3	TCACTTTCAAACGAGGTCCTC	TCCATACCCAGGCTCAATCT	47.6	50.0	57.9	57.8	729-730 bp	1.0	77.2
rps4-trnL-UAA_p4	TCACTTTCAAACGAGGTCCTC	TCTCCATACCCAGGCTCAAT	47.6	50.0	57.9	57.8	731-732 bp	1.0	77.2
rps4-trnL-UAA_p5	TCACTTTCAAACGAGGTCCT	CTGCCACTTGATACGTCTCC	45.0	55.0	56.7	58.1	746-747 bp	1.0	76.6