Hotspot

psbD-trnT-GGU

Genome · 33727-34994 · 1268 bp

Back to Phegopteris

Pi 0.0886
MarkerSeek score 81.00
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
Genome
length_bp
1268
variable_sites
111
pi
0.0886
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 TTTTCCAATGGCAATGGTCA TTCAAATCCGACAAAGGGCT 40.0 45.0 56.1 57.7 1301-1309 bp 1.0 70.2
psbD-trnT-GGU_p2 TTTTCCAATGGCAATGGTCA CCCCTTTAACTCAGCGGTAG 40.0 55.0 56.1 57.7 1352-1360 bp 1.0 69.7
psbD-trnT-GGU_p3 TTTTCCAATGGCAATGGTCA GTTCAAATCCGACAAAGGGC 40.0 50.0 56.1 57.7 1302-1310 bp 1.0 69.7
psbD-trnT-GGU_p4 TTTCCAATGGCAATGGTCAT TTCAAATCCGACAAAGGGCT 40.0 45.0 55.9 57.7 1300-1308 bp 1.0 68.3
psbD-trnT-GGU_p5 TTTCCAATGGCAATGGTCAT CCCCTTTAACTCAGCGGTAG 40.0 55.0 55.9 57.7 1351-1359 bp 1.0 67.8