Hotspot

psbM-petN

Genome · 28005-28923 · 919 bp

Back to Phegopteris

Pi 0.0931

MarkerSeek score 84.90

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 919
variable_sites: 82
pi: 0.0931
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCCCAACAGCTTTTCTGCTT	TCTCACTTGTAGTTCGGGGA	45.0	50.0	57.9	57.7	977-1010 bp	1.0	86.7
psbM-petN_p2	CGGCCGCTCAGAATAACTAG	TCTCACTTGTAGTTCGGGGA	55.0	50.0	58.2	57.7	941-974 bp	1.0	85.8
psbM-petN_p3	TCCCAACAGCTTTTCTGCTT	TTGTAGTTCGGGGAAGAAGC	45.0	50.0	57.9	57.5	971-1004 bp	1.0	85.4
psbM-petN_p4	ATCCCAACAGCTTTTCTGCT	TCTCACTTGTAGTTCGGGGA	45.0	50.0	57.7	57.7	978-1011 bp	1.0	84.9
psbM-petN_p5	CGGCCGCTCAGAATAACTAG	TTGTAGTTCGGGGAAGAAGC	55.0	50.0	58.2	57.5	935-968 bp	1.0	84.5