Hotspot

ycf1

SSC · 121799-126967 · 5169 bp

Back to Phedimus

Pi 0.0228

MarkerSeek score 54.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5169
variable_sites: 400
pi: 0.0228
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTTGTATGACCGTCGGGG	AAGAGGGATCCACCGAAGAA	50.0	50.0	58.0	58.0	907-919 bp	1.0	87.2
ycf1_p2	TCCTCTGGTTCTGCTTCGTA	AAGAGGGATCCACCGAAGAA	50.0	50.0	58.1	58.0	1051-1063 bp	1.0	87.0
ycf1_p3	AATTTGTATGACCGTCGGGG	CAGTAACGGAAGAAAGGGGG	50.0	55.0	58.0	57.9	994-1006 bp	1.0	86.1
ycf1_p4	CGAAAATCAGGTTGTTGGGC	AAGAGGGATCCACCGAAGAA	50.0	50.0	57.9	58.0	1102 bp	0.769231	70.9
ycf1_p5	ACCACCCCAAATCTTGCTTT	AAGAGGGATCCACCGAAGAA	45.0	50.0	57.9	58.0	1012 bp	0.769231	70.4