Hotspot

petA-psbJ

LSC · 61953-62943 · 991 bp

Back to Phedimus

Pi 0.0281

MarkerSeek score 70.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 991
variable_sites: 97
pi: 0.0281
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAAATCTTTTTGGTTCT	ACTGGTATTGCTGTGATCGG	36.4	50.0	57.2	57.7	917-1144 bp	1.0	66.6
petA-psbJ_p2	TGGCACAAATCTTTTTGGTTCT	CTGGTATTGCTGTGATCGGT	36.4	50.0	57.2	57.7	916-1143 bp	1.0	66.6
petA-psbJ_p3	TGGCACAAATCTTTTTGGTTCTT	ACTGGTATTGCTGTGATCGG	34.8	50.0	57.9	57.7	917-1144 bp	1.0	63.8
petA-psbJ_p4	TGGCACAAATCTTTTTGGTTCTT	CTGGTATTGCTGTGATCGGT	34.8	50.0	57.9	57.7	916-1143 bp	1.0	63.8
petA-psbJ_p5	TGGCACAAATCTTTTTGGTTCT	TGGTATTGCTGTGATCGGTT	36.4	45.0	57.2	57.2	915-1142 bp	1.0	62.9