Hotspot

ycf4-cemA

LSC · 59478-60080 · 603 bp

Back to Phedimus

Pi 0.0296

MarkerSeek score 64.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 603
variable_sites: 58
pi: 0.0296
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTAGCCTATTTCTTGCGCGT	TAAGCAGAAGTTCCCAACCG	45.0	50.0	58.0	57.8	1164-1175 bp	0.769231	70.5
ycf4-cemA_p2	TATGGTATCGCGGGTCTCTT	TAAGCAGAAGTTCCCAACCG	50.0	50.0	58.0	57.8	1476-1487 bp	0.769231	70.3
ycf4-cemA_p3	TTCTATGGTATCGCGGGTCT	TAAGCAGAAGTTCCCAACCG	50.0	50.0	58.0	57.8	1479-1490 bp	0.769231	70.3
ycf4-cemA_p4	GTTGGCGATCAGAACAGCTA	TAAGCAGAAGTTCCCAACCG	50.0	50.0	58.0	57.8	1676-1687 bp	0.769231	70.3
ycf4-cemA_p5	ATTTCGGTTGGGCCTGTATC	TAAGCAGAAGTTCCCAACCG	50.0	50.0	57.9	57.8	1619-1630 bp	0.769231	70.0