Hotspot

psbZ-trnG-GCC

LSC · 36073-36773 · 701 bp

Back to Phedimus

Pi 0.0189

MarkerSeek score 65.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 44
pi: 0.0189
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TCCGGTACATCGTTATGGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.2	57.8	738-843 bp	1.0	85.3
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	782-887 bp	1.0	85.2
psbZ-trnG-GCC_p3	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	780-885 bp	1.0	84.8
psbZ-trnG-GCC_p4	TCCGGTACATCGTTATGGGT	AAATCCATTGGGCGGATAGC	50.0	50.0	58.2	58.4	772-877 bp	1.0	83.4
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	AAATCCATTGGGCGGATAGC	50.0	50.0	57.8	58.4	816-921 bp	1.0	83.3