Hotspot

trnT-GGU-psbD

LSC · 32174-32875 · 702 bp

Back to Phedimus

Pi 0.0259

MarkerSeek score 63.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 57
pi: 0.0259
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CATAGTACCCTACCCCCAGG	AAACTAGGCGCAAAGAACCA	60.0	45.0	58.0	58.0	1650-2236 bp	1.0	85.6
trnT-GGU-psbD_p2	CATAGTACCCTACCCCCAGG	AACTAGGCGCAAAGAACCAA	60.0	45.0	58.0	58.0	1649-2235 bp	1.0	85.6
trnT-GGU-psbD_p3	CATAGTACCCTACCCCCAGG	ACCAAAACTAGGCGCAAAGA	60.0	45.0	58.0	58.0	1654-2240 bp	1.0	85.6
trnT-GGU-psbD_p4	CATAGTACCCTACCCCCAGG	CGCCTAATACACCTGCAACT	60.0	50.0	58.0	58.0	1751-2337 bp	1.0	85.3
trnT-GGU-psbD_p5	CATAGTACCCTACCCCCAGG	ACAAAAACAGCAATTGGCCC	60.0	45.0	58.0	58.0	1611-1621 bp	0.692308	68.0