Hotspot

trnE-UUC-trnT-GGU

LSC · 31812-32099 · 288 bp

Back to Phedimus

Pi 0.031

MarkerSeek score 50.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 27
pi: 0.031
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	428-988 bp	1.0	84.2
trnE-UUC-trnT-GGU_p2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	407-967 bp	1.0	83.9
trnE-UUC-trnT-GGU_p3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	58.0	58.1	404-964 bp	1.0	83.8
trnE-UUC-trnT-GGU_p4	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	425-985 bp	1.0	83.1
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	393-953 bp	1.0	82.6