Hotspot

trnG-GCC-trnR-UCU

LSC · 10149-10461 · 313 bp

Back to Phedimus

Pi 0.0596

MarkerSeek score 67.80

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 63
pi: 0.0596
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TCCTGAGTTGACGTCGACTA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.8	55.6	412-438 bp	1.0	55.6
trnG-GCC-trnR-UCU_p2	TCCTGAGTTGACGTCGACTA	AGAAGACCTATGTCCTATCCA	50.0	42.9	57.8	54.6	406-432 bp	1.0	55.4
trnG-GCC-trnR-UCU_p3	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.2	55.6	385-411 bp	1.0	55.3
trnG-GCC-trnR-UCU_p4	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.2	54.6	379-405 bp	1.0	55.1
trnG-GCC-trnR-UCU_p5	TCCTGAGTTGACGTCGACTA	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	57.8	57.6	417-443 bp	1.0	55.0