Hotspot

rps16-trnQ-UUG

LSC · 5909-7421 · 1513 bp

Back to Phedimus

Pi 0.0291
MarkerSeek score 68.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1513
variable_sites
160
pi
0.0291
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GGTTCGAATCCTTCCGTCC 45.0 57.9 58.1 57.9 1309-1601 bp 1.0 82.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTCCC 45.0 57.9 58.1 57.9 1308-1600 bp 1.0 82.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGAATCCTTCCGTCCCA 45.0 52.6 58.1 58.3 1307-1599 bp 1.0 80.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA AGGTTCGAATCCTTCCGTCC 45.0 55.0 58.1 59.5 1310-1602 bp 1.0 79.2
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGAATCCTTCCGTCCCAG 45.0 55.0 58.1 59.5 1307-1599 bp 1.0 79.2