Hotspot

psbA

LSC · 118-1179 · 1062 bp

Back to Phebalium

Pi 0.0032

MarkerSeek score 46.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 8
pi: 0.0032
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGGTCGGTATGGATCCGTTA	ACATAGGGAAAGCTGTGTGC	55.0	50.0	58.7	58.2	1512-1563 bp	1.0	76.2
psbA_p2	GGGTCGGTATGGATCCGTTA	CATAGGGAAAGCTGTGTGCA	55.0	50.0	58.7	58.2	1511-1562 bp	1.0	76.2
psbA_p3	GGGTCGGTATGGATCCGTTA	AGGGCGGATTTGGTATTTGG	55.0	50.0	58.7	58.2	1769-1820 bp	1.0	75.4
psbA_p4	GGGTCGGTATGGATCCGTTA	GGGCGGATTTGGTATTTGGA	55.0	50.0	58.7	58.2	1768-1819 bp	1.0	75.4
psbA_p5	GGGTCGGTATGGATCCGTTA	TATGCTTGGGAGTCCCTGAT	55.0	50.0	58.7	57.8	1160-1176 bp	0.8	66.8