Hotspot

trnV-GAC-rps12

IRa · 139780-141555 · 1776 bp

Back to Phebalium

Pi 0.00327
MarkerSeek score 61.30
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1776
variable_sites
11
pi
0.00327
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1860-1872 bp 1.0 77.7
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1864-1876 bp 1.0 77.6
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1859-1871 bp 1.0 77.6
trnV-GAC-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1862-1874 bp 1.0 76.7
trnV-GAC-rps12_p5 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1845-1857 bp 1.0 76.2