Hotspot

rps15-ycf1

SSC · 124954-125319 · 366 bp

Back to Phebalium

Pi 0.00992

MarkerSeek score 47.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 366
variable_sites: 8
pi: 0.00992
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GCTGGCTCTTTTGGGTACTT	TGCTTATTATCGAACCCCGC	50.0	50.0	58.1	58.1	1560-1567 bp	1.0	80.3
rps15-ycf1_p2	CCAACGGGTTACATAAGGCA	TGCTTATTATCGAACCCCGC	50.0	50.0	57.9	58.1	1473-1480 bp	1.0	80.2
rps15-ycf1_p3	ATTTTCCGTAGCCCCCTTTG	TGCTTATTATCGAACCCCGC	50.0	50.0	58.2	58.1	1059-1063 bp	1.0	80.0
rps15-ycf1_p4	GCTGGCTCTTTTGGGTACTT	ATTGTCACCTGGTTCGACTG	50.0	50.0	58.1	57.8	1689-1696 bp	1.0	79.9
rps15-ycf1_p5	CCAACGGGTTACATAAGGCA	ATTGTCACCTGGTTCGACTG	50.0	50.0	57.9	57.8	1602-1609 bp	1.0	79.8