Hotspot

rpl32-trnL-UAG

SSC · 114938-115521 · 584 bp

Back to Phebalium

Pi 0.0125

MarkerSeek score 54.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 584
variable_sites: 15
pi: 0.0125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTTGCGGTCGGAAAAAGTA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1289-1317 bp	1.0	82.0
rpl32-trnL-UAG_p2	TGTTGCGGTCGGAAAAAGTA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1290-1318 bp	1.0	82.0
rpl32-trnL-UAG_p3	AGCCCCACCCCTATTAACAT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1267-1295 bp	1.0	81.8
rpl32-trnL-UAG_p4	TGTTGCGGTCGGAAAAAGTA	TGCCTCGAGTAAATCCAACG	45.0	50.0	58.0	58.0	1649-1677 bp	1.0	81.6
rpl32-trnL-UAG_p5	AGCCCCACCCCTATTAACAT	TGCCTCGAGTAAATCCAACG	50.0	50.0	58.1	58.0	1627-1655 bp	1.0	81.4