Hotspot

rps18-rpl20

LSC · 69765-69958 · 194 bp

Back to Phebalium

Pi 0.00722

MarkerSeek score 59.60

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 3
pi: 0.00722
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GAAAGAAGGGGGTCGATCAC	CTCACGGAGTTCTCTGGAGA	55.0	55.0	58.0	58.2	272 bp	0.4	54.7
rps18-rpl20_p2	GAAGGGGGTCGATCACTAGA	CTCACGGAGTTCTCTGGAGA	55.0	55.0	57.9	58.2	268 bp	0.4	54.4
rps18-rpl20_p3	AGAAGGGGGTCGATCACTAG	CTCACGGAGTTCTCTGGAGA	55.0	55.0	57.9	58.2	269 bp	0.4	54.3
rps18-rpl20_p4	GAACTCCAGGTCTTCGAACC	CTCACGGAGTTCTCTGGAGA	55.0	55.0	57.9	58.2	250 bp	0.4	54.3
rps18-rpl20_p5	GAAAGAAGGGGGTCGATCAC	TCACGGAGTTCTCTGGAGAA	55.0	50.0	58.0	57.7	271 bp	0.4	53.8