Hotspot

psbZ-trnG-GCC

LSC · 37317-37857 · 541 bp

Back to Phebalium

Pi 0.0233
MarkerSeek score 66.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
541
variable_sites
23
pi
0.0233
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CGGATGGTTGGTCGAGTAAC TCTTCTCCTTGGCAAAGAGA 55.0 45.0 58.4 56.1 665-687 bp 1.0 67.0
psbZ-trnG-GCC_p2 TTTGCTTCTCCGGATGGTTG TCTTCTCCTTGGCAAAGAGA 50.0 45.0 58.5 56.1 675-697 bp 1.0 66.1
psbZ-trnG-GCC_p3 ATTTGCTTCTCCGGATGGTT TCTTCTCCTTGGCAAAGAGA 45.0 45.0 57.5 56.1 676-698 bp 1.0 65.7
psbZ-trnG-GCC_p4 CGGATGGTTGGTCGAGTAAC TCTTCTCCTTGGCAAAGAGAA 55.0 42.9 58.4 56.9 665-687 bp 1.0 65.2
psbZ-trnG-GCC_p5 TTTGCTTCTCCGGATGGTTG TCTTCTCCTTGGCAAAGAGAA 50.0 42.9 58.5 56.9 675-697 bp 1.0 64.3