Hotspot

trnC-GCA-petN

LSC · 28420-29077 · 658 bp

Back to Phebalium

Pi 0.00894

MarkerSeek score 62.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 13
pi: 0.00894
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.4	58.1	769-796 bp	1.0	80.7
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	731-758 bp	1.0	80.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CCATACTACGAGCGAAAGGG	50.0	55.0	58.4	57.9	776-803 bp	1.0	80.4
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CCCATACTACGAGCGAAAGG	50.0	55.0	58.4	57.9	777-804 bp	1.0	80.4
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CCCCATACTACGAGCGAAAG	50.0	55.0	58.4	57.9	778-805 bp	1.0	80.4