Hotspot

atpF-atpH

LSC · 12902-13381 · 480 bp

Back to Phebalium

Pi 0.0212

MarkerSeek score 66.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 21
pi: 0.0212
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCAGTGAACCAAAGAAACG	TGGACTGGTTGTAGCATTAGC	50.0	47.6	57.9	58.0	538-579 bp	1.0	77.9
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGACTGGTTGTAGCATTAGC	50.0	47.6	57.9	58.0	564-605 bp	1.0	77.8
atpF-atpH_p3	TGGCCAGTGAACCAAAGAAA	TGGACTGGTTGTAGCATTAGC	45.0	47.6	57.8	58.0	540-581 bp	1.0	77.7
atpF-atpH_p4	AATGGCCAGTGAACCAAAGA	TGGACTGGTTGTAGCATTAGC	45.0	47.6	57.6	58.0	542-583 bp	1.0	75.8
atpF-atpH_p5	ATGGCCAGTGAACCAAAGAA	TGGACTGGTTGTAGCATTAGC	45.0	47.6	57.6	58.0	541-582 bp	1.0	75.8