Hotspot

trnS-GCU-trnG-UCC

LSC · 7922-8722 · 801 bp

Back to Phebalium

Pi 0.0111

MarkerSeek score 58.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 17
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.0	57.2	879-911 bp	1.0	59.0
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	GGGGATTACTTGAACGAATCAC	50.0	45.5	58.0	57.1	878-910 bp	1.0	58.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.2	57.2	937-969 bp	1.0	57.7
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	GGGGATTACTTGAACGAATCAC	50.0	45.5	58.2	57.1	936-968 bp	1.0	57.4
trnS-GCU-trnG-UCC_p5	ACGCTTTAATCCACTCAGCC	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.3	57.2	876-908 bp	1.0	56.9