Hotspot

ycf1

SSC · 118948-124278 · 5331 bp

Back to Phaseolus

Pi 0.0208

MarkerSeek score 52.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5331
variable_sites: 110
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTTGTGACAAAAAGGAGACA	TGCACATATAACGGCGTTCA	42.9	45.0	56.9	58.0	1028-1061 bp	1.0	68.1
ycf1_p2	TGCTTGTGACAAAAAGGAGAC	TGCACATATAACGGCGTTCA	42.9	45.0	56.9	58.0	1029-1062 bp	1.0	68.1
ycf1_p3	GCTTGTGACAAAAAGGAGACA	AACGGCGTTCAATTATCCGA	42.9	45.0	56.9	58.0	1019-1052 bp	1.0	68.1
ycf1_p4	GCTTGTGACAAAAAGGAGACA	ACGGCGTTCAATTATCCGAA	42.9	45.0	56.9	58.0	1018-1051 bp	1.0	68.1
ycf1_p5	TGCTTGTGACAAAAAGGAGAC	ACGGCGTTCAATTATCCGAA	42.9	45.0	56.9	58.0	1019-1052 bp	1.0	68.1