Hotspot

ccsA-ndhD

SSC · 110372-110626 · 255 bp

Back to Phaseolus

Pi 0.0431

MarkerSeek score 69.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 255
variable_sites: 11
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCTTCGATAGTGGCCTCCAT	TTTCGGCTGACAAGGTTGAA	50.0	45.0	57.9	58.0	407-412 bp	1.0	81.1
ccsA-ndhD_p2	TCTTCGATAGTGGCCTCCAT	TTTTCGGCTGACAAGGTTGA	50.0	45.0	57.9	58.0	408-413 bp	1.0	81.1
ccsA-ndhD_p3	TTCTTCGATAGTGGCCTCCA	TTTCGGCTGACAAGGTTGAA	50.0	45.0	58.1	58.0	408-413 bp	1.0	80.7
ccsA-ndhD_p4	TTCTTCGATAGTGGCCTCCA	TTTTCGGCTGACAAGGTTGA	50.0	45.0	58.1	58.0	409-414 bp	1.0	80.7
ccsA-ndhD_p5	CTTCGATAGTGGCCTCCATG	TTTCGGCTGACAAGGTTGAA	55.0	45.0	57.8	58.0	406-411 bp	1.0	80.5