Hotspot

psaJ-rps18

LSC · 66034-66738 · 705 bp

Back to Phaseolus

Pi 0.0128
MarkerSeek score 66.70
Primer available yes
Label psaJ-rps18

Feature Metadata

label_name
psaJ-rps18
feature_type
igs
region
LSC
length_bp
705
variable_sites
9
pi
0.0128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rps18_p1 CATATCTTTCCGTGGCACCA GGGCAAACGTCTACGAAAAG 50.0 50.0 58.0 57.4 884-952 bp 1.0 76.1
psaJ-rps18_p2 ACATATCTTTCCGTGGCACC GGGCAAACGTCTACGAAAAG 50.0 50.0 58.0 57.4 885-953 bp 1.0 76.1
psaJ-rps18_p3 CATATCTTTCCGTGGCACCA GGGGCAAACGTCTACGAAAA 50.0 50.0 58.0 58.8 885-953 bp 1.0 74.7
psaJ-rps18_p4 ACATATCTTTCCGTGGCACC GGGGCAAACGTCTACGAAAA 50.0 50.0 58.0 58.8 886-954 bp 1.0 74.7
psaJ-rps18_p5 GCTTTGGCGGGTCTATTGAT GGGCAAACGTCTACGAAAAG 50.0 50.0 58.3 57.4 840-908 bp 1.0 73.9