Hotspot

psbI-psbK

LSC · 55522-55988 · 467 bp

Back to Phaseolus

Pi 0.0257

MarkerSeek score 68.40

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 12
pi: 0.0257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	ACGCCCTGGATCGTTAGATA	CTTTTGTTTGGCAAGCTGCT	50.0	45.0	58.0	58.1	592 bp	1.0	81.1
psbI-psbK_p2	TACGCCCTGGATCGTTAGAT	CTTTTGTTTGGCAAGCTGCT	50.0	45.0	58.0	58.1	593 bp	1.0	81.1
psbI-psbK_p3	ACGCCCTGGATCGTTAGATA	GTTTGGCAAGCTGCTGTAAG	50.0	50.0	58.0	57.9	587 bp	1.0	81.1
psbI-psbK_p4	ACGCCCTGGATCGTTAGATA	GAGAAATTGCCCGAAGCCTA	50.0	50.0	58.0	58.0	674 bp	1.0	81.0
psbI-psbK_p5	TACGCCCTGGATCGTTAGAT	GAGAAATTGCCCGAAGCCTA	50.0	50.0	58.0	58.0	675 bp	1.0	81.0