Hotspot

petN-trnC-GCA

LSC · 34995-35465 · 471 bp

Back to Phaseolus

Pi 0.0325

MarkerSeek score 66.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 15
pi: 0.0325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AAAGCAGCCCAAGCAATACT	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.1	58.1	523-532 bp	1.0	82.4
petN-trnC-GCA_p2	AAGCAGCCCAAGCAATACTT	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.1	58.1	522-531 bp	1.0	82.4
petN-trnC-GCA_p3	AAAGCAGCCCAAGCAATACT	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.1	58.4	541-550 bp	1.0	80.1
petN-trnC-GCA_p4	AAGCAGCCCAAGCAATACTT	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.1	58.4	540-549 bp	1.0	80.1
petN-trnC-GCA_p5	CATTAAAGCAGCCCAAGCAA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	57.3	58.1	527-536 bp	1.0	77.0