Hotspot

trnS-UGA-psbC

LSC · 28562-28760 · 199 bp

Back to Phaseolus

Pi 0.0754

MarkerSeek score 78.30

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 15
pi: 0.0754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TCGCGATTTTGAACCTGTTC	55.0	45.0	58.0	57.0	334 bp	1.0	75.7
trnS-UGA-psbC_p2	GAGAGATGGCTGAGTGGTTG	CGCGATTTTGAACCTGTTCT	55.0	45.0	58.0	57.0	333 bp	1.0	75.7
trnS-UGA-psbC_p3	ATAGCCCCGGTCTTGAAAAC	TCGCGATTTTGAACCTGTTC	50.0	45.0	57.9	57.0	314 bp	1.0	75.1
trnS-UGA-psbC_p4	GCTTTTGGAGAGATGGCTGA	TCGCGATTTTGAACCTGTTC	50.0	45.0	57.9	57.0	341 bp	1.0	74.9
trnS-UGA-psbC_p5	AGCTTTTGGAGAGATGGCTG	TCGCGATTTTGAACCTGTTC	50.0	45.0	57.9	57.0	342 bp	1.0	74.8