Hotspot

psbZ-trnS-UGA

LSC · 28103-28468 · 366 bp

Back to Phaseolus

Pi 0.0109
MarkerSeek score 53.90
Primer available yes
Label psbZ-trnS-UGA

Feature Metadata

label_name
psbZ-trnS-UGA
feature_type
igs
region
LSC
length_bp
366
variable_sites
4
pi
0.0109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnS-UGA_p1 TTGACCAACCTTCAGGAGAA CAACCACTCAGCCATCTCTC 45.0 55.0 56.3 58.0 490-495 bp 1.0 66.8
psbZ-trnS-UGA_p2 TTTGACCAACCTTCAGGAGA CAACCACTCAGCCATCTCTC 45.0 55.0 56.3 58.0 491-496 bp 1.0 66.8
psbZ-trnS-UGA_p3 TTGACCAACCTTCAGGAGAA GTTTTCAAGACCGGGGCTAT 45.0 50.0 56.3 57.9 510-515 bp 1.0 65.9
psbZ-trnS-UGA_p4 TTTGACCAACCTTCAGGAGA GTTTTCAAGACCGGGGCTAT 45.0 50.0 56.3 57.9 511-516 bp 1.0 65.9
psbZ-trnS-UGA_p5 TTTGACCAACCTTCAGGAGAA CAACCACTCAGCCATCTCTC 42.9 55.0 57.1 58.0 491-496 bp 1.0 64.9