Hotspot

ycf3-psaA

LSC · 21129-21881 · 753 bp

Back to Phaseolus

Pi 0.0161

MarkerSeek score 68.70

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 12
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AAGTTGCGGAGTCTTGGTTT	AATGACCAGGTTTTGCCCAT	45.0	45.0	57.9	58.0	963-966 bp	1.0	80.8
ycf3-psaA_p2	AAGTTGCGGAGTCTTGGTTT	ATGACCAGGTTTTGCCCATT	45.0	45.0	57.9	58.0	962-965 bp	1.0	80.8
ycf3-psaA_p3	TTGGTTGAAGATCACAGGGC	AATGACCAGGTTTTGCCCAT	50.0	45.0	58.1	58.0	875-878 bp	1.0	80.7
ycf3-psaA_p4	TTGGTTGAAGATCACAGGGC	ATGACCAGGTTTTGCCCATT	50.0	45.0	58.1	58.0	874-877 bp	1.0	80.7
ycf3-psaA_p5	AGCTCTTACCCCCGGTAATT	ATGACCAGGTTTTGCCCATT	50.0	45.0	58.1	58.0	910-913 bp	1.0	80.5