Hotspot

rps3-rpl16

LSC · 676-824 · 149 bp

Back to Phaseolus

Pi 0.0537

MarkerSeek score 69.70

Primer available yes

Label rps3-rpl16

Feature Metadata

label_name: rps3-rpl16
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 8
pi: 0.0537
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl16_p1	TGGGATTTTAGGGATAAAAGTTTGG	AACGAGTCACACACTAAGCA	36.0	45.0	57.5	57.1	210-214 bp	1.0	43.0
rps3-rpl16_p2	ACAGTTCGAACTATTTATGGGA	AACGAGTCACACACTAAGCA	36.4	45.0	54.9	57.1	227-231 bp	1.0	42.8
rps3-rpl16_p3	ACAGTTCGAACTATTTATGGGA	ACGAGTCACACACTAAGCAT	36.4	45.0	54.9	56.9	226-230 bp	1.0	42.8
rps3-rpl16_p4	ACAGTTCGAACTATTTATGGGAT	AACGAGTCACACACTAAGCA	34.8	45.0	55.5	57.1	227-231 bp	1.0	42.8
rps3-rpl16_p5	ACAGTTCGAACTATTTATGGGA	AACGAGTCACACACTAAGCAT	36.4	42.9	54.9	57.6	227-231 bp	1.0	42.8