primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl16_p1	rps3-rpl16	1	TGGGATTTTAGGGATAAAAGTTTGG	AACGAGTCACACACTAAGCA	25	20	36.0	45.0	57.5	57.1	0.0	0.0	6.372166	676	824	210-214	1.000000	43.0
rps3-rpl16_p2	rps3-rpl16	2	ACAGTTCGAACTATTTATGGGA	AACGAGTCACACACTAAGCA	22	20	36.4	45.0	54.9	57.1	29.3	0.0	5.966520	676	824	227-231	1.000000	42.8
rps3-rpl16_p3	rps3-rpl16	3	ACAGTTCGAACTATTTATGGGA	ACGAGTCACACACTAAGCAT	22	20	36.4	45.0	54.9	56.9	29.3	0.0	6.192807	676	824	226-230	1.000000	42.8
rps3-rpl16_p4	rps3-rpl16	4	ACAGTTCGAACTATTTATGGGAT	AACGAGTCACACACTAAGCA	23	20	34.8	45.0	55.5	57.1	29.3	0.0	6.414239	676	824	227-231	1.000000	42.8
rps3-rpl16_p5	rps3-rpl16	5	ACAGTTCGAACTATTTATGGGA	AACGAGTCACACACTAAGCAT	22	21	36.4	42.9	54.9	57.6	29.3	0.0	6.470361	676	824	227-231	1.000000	42.8
ycf3-psaA_p1	ycf3-psaA	1	AAGTTGCGGAGTCTTGGTTT	AATGACCAGGTTTTGCCCAT	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.081344	21129	21881	963-966	1.000000	80.8
ycf3-psaA_p2	ycf3-psaA	2	AAGTTGCGGAGTCTTGGTTT	ATGACCAGGTTTTGCCCATT	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.081344	21129	21881	962-965	1.000000	80.8
ycf3-psaA_p3	ycf3-psaA	3	TTGGTTGAAGATCACAGGGC	AATGACCAGGTTTTGCCCAT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.118862	21129	21881	875-878	1.000000	80.7
ycf3-psaA_p4	ycf3-psaA	4	TTGGTTGAAGATCACAGGGC	ATGACCAGGTTTTGCCCATT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.118862	21129	21881	874-877	1.000000	80.7
ycf3-psaA_p5	ycf3-psaA	5	AGCTCTTACCCCCGGTAATT	ATGACCAGGTTTTGCCCATT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.139388	21129	21881	910-913	1.000000	80.5
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGAAGGTTGGTCA	20	20	50.0	50.0	57.8	56.8	1.1	0.0	1.400314	27218	27913	823-850	1.000000	73.2
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGAAGGTTGGTCAA	20	21	50.0	47.6	57.8	57.5	1.1	0.0	1.661934	27218	27913	823-850	1.000000	71.1
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	CGTCTTCTCCTTGGCAAAGA	TCTCCTGAAGGTTGGTCAAA	20	20	50.0	45.0	57.8	56.3	1.1	0.0	1.883910	27218	27913	821-848	1.000000	69.3
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	CGTCTTCTCCTTGGCAAAGA	TTCTCCTGAAGGTTGGTCAA	20	20	50.0	45.0	57.8	56.3	1.1	0.0	1.883910	27218	27913	822-849	1.000000	69.3
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	CGTCTTCTCCTTGGCAAAGA	TTCTCCTGAAGGTTGGTCAAA	20	21	50.0	42.9	57.8	57.1	1.1	0.0	2.113503	27218	27913	822-849	1.000000	67.5
psbZ-trnS-UGA_p1	psbZ-trnS-UGA	1	TTGACCAACCTTCAGGAGAA	CAACCACTCAGCCATCTCTC	20	20	45.0	55.0	56.3	58.0	0.0	0.0	1.735432	28103	28468	490-495	1.000000	66.8
psbZ-trnS-UGA_p2	psbZ-trnS-UGA	2	TTTGACCAACCTTCAGGAGA	CAACCACTCAGCCATCTCTC	20	20	45.0	55.0	56.3	58.0	0.0	0.0	1.735432	28103	28468	491-496	1.000000	66.8
psbZ-trnS-UGA_p3	psbZ-trnS-UGA	3	TTGACCAACCTTCAGGAGAA	GTTTTCAAGACCGGGGCTAT	20	20	45.0	50.0	56.3	57.9	0.0	0.8	1.838354	28103	28468	510-515	1.000000	65.9
psbZ-trnS-UGA_p4	psbZ-trnS-UGA	4	TTTGACCAACCTTCAGGAGA	GTTTTCAAGACCGGGGCTAT	20	20	45.0	50.0	56.3	57.9	0.0	0.8	1.838354	28103	28468	511-516	1.000000	65.9
psbZ-trnS-UGA_p5	psbZ-trnS-UGA	5	TTTGACCAACCTTCAGGAGAA	CAACCACTCAGCCATCTCTC	21	20	42.9	55.0	57.1	58.0	0.0	0.0	1.965026	28103	28468	491-496	1.000000	64.9
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	GAGAGATGGCTGAGTGGTTG	TCGCGATTTTGAACCTGTTC	20	20	55.0	45.0	58.0	57.0	0.0	3.7	0.982877	28562	28760	334-334	1.000000	75.7
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GAGAGATGGCTGAGTGGTTG	CGCGATTTTGAACCTGTTCT	20	20	55.0	45.0	58.0	57.0	0.0	0.0	0.990798	28562	28760	333-333	1.000000	75.7
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	ATAGCCCCGGTCTTGAAAAC	TCGCGATTTTGAACCTGTTC	20	20	50.0	45.0	57.9	57.0	0.0	3.7	1.085799	28562	28760	314-314	1.000000	75.1
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GCTTTTGGAGAGATGGCTGA	TCGCGATTTTGAACCTGTTC	20	20	50.0	45.0	57.9	57.0	0.0	3.7	1.081323	28562	28760	341-341	1.000000	74.9
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	AGCTTTTGGAGAGATGGCTG	TCGCGATTTTGAACCTGTTC	20	20	50.0	45.0	57.9	57.0	0.0	3.7	1.087295	28562	28760	342-342	1.000000	74.8
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	CCGCAACCAGTCATCCATAA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.0	58.1	0.0	7.6	0.101025	31192	32186	1125-1143	1.000000	81.9
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CCGCAACCAGTCATCCATAA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.123180	31192	32186	1128-1146	1.000000	81.8
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.233981	31192	32186	1146-1164	1.000000	80.8
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.233981	31192	32186	1147-1165	1.000000	80.8
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.233981	31192	32186	1148-1166	1.000000	80.8
petN-trnC-GCA_p1	petN-trnC-GCA	1	AAAGCAGCCCAAGCAATACT	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.153260	34995	35465	523-532	1.000000	82.4
petN-trnC-GCA_p2	petN-trnC-GCA	2	AAGCAGCCCAAGCAATACTT	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.153260	34995	35465	522-531	1.000000	82.4
petN-trnC-GCA_p3	petN-trnC-GCA	3	AAAGCAGCCCAAGCAATACT	GGGGGACTGCAAATCCTTTT	20	20	45.0	50.0	58.1	58.4	0.0	14.7	0.426623	34995	35465	541-550	1.000000	80.1
petN-trnC-GCA_p4	petN-trnC-GCA	4	AAGCAGCCCAAGCAATACTT	GGGGGACTGCAAATCCTTTT	20	20	45.0	50.0	58.1	58.4	0.0	14.7	0.426623	34995	35465	540-549	1.000000	80.1
petN-trnC-GCA_p5	petN-trnC-GCA	5	CATTAAAGCAGCCCAAGCAA	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	57.3	58.1	0.0	0.0	0.829524	34995	35465	527-536	1.000000	77.0
rpoC1-rpoC2_p1	rpoC1-rpoC2	1	TTTTTGCCGGGCCTATTCAT	TGGCAGTTCCACCTATCACT	20	20	45.0	50.0	58.1	58.3	18.0	0.0	0.484111	42634	42832	285-287	1.000000	78.4
rpoC1-rpoC2_p2	rpoC1-rpoC2	2	TTTTTGCCGGGCCTATTCAT	TTAGATTGGTCCGTTCTGCC	20	20	45.0	50.0	58.1	57.6	18.0	4.1	0.537288	42634	42832	252-254	1.000000	78.2
rpoC1-rpoC2_p3	rpoC1-rpoC2	3	TTGCCGGGCCTATTCATATG	TGGCAGTTCCACCTATCACT	20	20	50.0	50.0	57.8	58.3	18.0	0.0	0.542240	42634	42832	282-284	1.000000	77.9
rpoC1-rpoC2_p4	rpoC1-rpoC2	4	TTGCCGGGCCTATTCATATG	TTAGATTGGTCCGTTCTGCC	20	20	50.0	50.0	57.8	57.6	18.0	4.1	0.595417	42634	42832	249-251	1.000000	77.8
rpoC1-rpoC2_p5	rpoC1-rpoC2	5	GCTGTACAAGGTTTTTGCCG	TGGCAGTTCCACCTATCACT	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.577344	42634	42832	296-298	1.000000	77.5
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	TCCATGAAACGAATCGCACT	ATCCGACGCTATAGTCCACT	20	20	45.0	50.0	57.9	57.7	1.2	0.8	0.366844	54566	55185	684-695	1.000000	81.4
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	TCCATGAAACGAATCGCACT	GCAATCCGACGCTATAGTCC	20	20	45.0	55.0	57.9	58.3	1.2	0.8	0.393963	54566	55185	687-698	1.000000	81.1
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	GTCCATGAAACGAATCGCAC	ATCCGACGCTATAGTCCACT	20	20	50.0	50.0	57.8	57.7	1.2	0.8	0.433005	54566	55185	685-696	1.000000	80.8
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	GTCCATGAAACGAATCGCAC	GCAATCCGACGCTATAGTCC	20	20	50.0	55.0	57.8	58.3	1.2	0.8	0.460124	54566	55185	688-699	1.000000	80.6
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	TCCATGAAACGAATCGCACT	TCCGACGCTATAGTCCACTC	20	20	45.0	55.0	57.9	58.4	1.2	0.8	0.502374	54566	55185	683-694	1.000000	80.3
psbI-psbK_p1	psbI-psbK	1	ACGCCCTGGATCGTTAGATA	CTTTTGTTTGGCAAGCTGCT	20	20	50.0	45.0	58.0	58.1	7.9	15.2	0.064847	55522	55988	592-592	1.000000	81.1
psbI-psbK_p2	psbI-psbK	2	TACGCCCTGGATCGTTAGAT	CTTTTGTTTGGCAAGCTGCT	20	20	50.0	45.0	58.0	58.1	16.5	15.2	0.064847	55522	55988	593-593	1.000000	81.1
psbI-psbK_p3	psbI-psbK	3	ACGCCCTGGATCGTTAGATA	GTTTGGCAAGCTGCTGTAAG	20	20	50.0	50.0	58.0	57.9	7.9	1.4	0.064973	55522	55988	587-587	1.000000	81.1
psbI-psbK_p4	psbI-psbK	4	ACGCCCTGGATCGTTAGATA	GAGAAATTGCCCGAAGCCTA	20	20	50.0	50.0	58.0	58.0	7.9	0.0	0.047266	55522	55988	674-674	1.000000	81.0
psbI-psbK_p5	psbI-psbK	5	TACGCCCTGGATCGTTAGAT	GAGAAATTGCCCGAAGCCTA	20	20	50.0	50.0	58.0	58.0	16.5	0.0	0.047266	55522	55988	675-675	1.000000	81.0
accD_p1	accD	1	ACTCCCCGCTCTCTTGTTAT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.159640	57764	59230	2218-2264	1.000000	83.9
accD_p2	accD	2	ACTCCCCGCTCTCTTGTTAT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.1	57.9	0.0	26.8	0.236174	57764	59230	2224-2270	1.000000	83.3
accD_p3	accD	3	ACTCCCCGCTCTCTTGTTAT	ATTTTTCCCTACTGACCGCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.256914	57764	59230	2283-2329	1.000000	83.1
accD_p4	accD	4	ACTCCCCGCTCTCTTGTTAT	TGCAACCAACAATAACCCCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.220741	57764	59230	2592-2641	1.000000	83.0
accD_p5	accD	5	ACTCCCCGCTCTCTTGTTAT	TTCCCTACTGACCGCCATAT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.229747	57764	59230	2325-2325	0.500000	58.2
psaJ-rps18_p1	psaJ-rps18	1	CATATCTTTCCGTGGCACCA	GGGCAAACGTCTACGAAAAG	20	20	50.0	50.0	58.0	57.4	12.9	9.9	0.626295	66034	66738	884-952	1.000000	76.1
psaJ-rps18_p2	psaJ-rps18	2	ACATATCTTTCCGTGGCACC	GGGCAAACGTCTACGAAAAG	20	20	50.0	50.0	58.0	57.4	12.9	9.9	0.627724	66034	66738	885-953	1.000000	76.1
psaJ-rps18_p3	psaJ-rps18	3	CATATCTTTCCGTGGCACCA	GGGGCAAACGTCTACGAAAA	20	20	50.0	50.0	58.0	58.8	12.9	9.9	0.808425	66034	66738	885-953	1.000000	74.7
psaJ-rps18_p4	psaJ-rps18	4	ACATATCTTTCCGTGGCACC	GGGGCAAACGTCTACGAAAA	20	20	50.0	50.0	58.0	58.8	12.9	9.9	0.809854	66034	66738	886-954	1.000000	74.7
psaJ-rps18_p5	psaJ-rps18	5	GCTTTGGCGGGTCTATTGAT	GGGCAAACGTCTACGAAAAG	20	20	50.0	50.0	58.3	57.4	0.0	9.9	0.909000	66034	66738	840-908	1.000000	73.9
rps11-rpl36_p1	rps11-rpl36	1	TTCCGCGAACCAATACGTAC	ATTGATTCGAAGACGGGGAC	20	20	50.0	50.0	58.4	57.7	8.2	3.3	0.683245	78284	78610	444-456	1.000000	79.0
rps11-rpl36_p2	rps11-rpl36	2	CCGCGAACCAATACGTACAT	ATTGATTCGAAGACGGGGAC	20	20	50.0	50.0	58.4	57.7	8.2	3.3	0.748013	78284	78610	442-454	1.000000	78.5
rps11-rpl36_p3	rps11-rpl36	3	ATTCCGCGAACCAATACGTA	ATTGATTCGAAGACGGGGAC	20	20	45.0	50.0	57.4	57.7	8.2	3.3	0.893852	78284	78610	445-457	1.000000	77.3
rps11-rpl36_p4	rps11-rpl36	4	TTCCGCGAACCAATACGTAC	GATTGATTCGAAGACGGGGA	20	20	50.0	50.0	58.4	57.4	8.2	3.3	0.969025	78284	78610	445-457	1.000000	76.7
rps11-rpl36_p5	rps11-rpl36	5	GATTCCGCGAACCAATACGT	ATTGATTCGAAGACGGGGAC	20	20	50.0	50.0	58.7	57.7	8.2	3.3	1.027388	78284	78610	446-458	1.000000	76.2
rpl36-rps8_p1	rpl36-rps8	1	GAAATCACCCGACCTCGTAC	TCGAATTTTAGGGGGCATGG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.147304	78725	79206	1184-1193	1.000000	82.0
rpl36-rps8_p2	rpl36-rps8	2	GAAATCACCCGACCTCGTAC	TTACCATGGGGAAGGACACT	20	20	55.0	50.0	58.1	57.9	0.0	18.1	0.135152	78725	79206	1479-1488	1.000000	81.7
rpl36-rps8_p3	rpl36-rps8	3	CGAGACCAGGCCCTTTTATC	TTACCATGGGGAAGGACACT	20	20	55.0	50.0	58.0	57.9	0.0	18.1	0.098820	78725	79206	1634-1634	0.500000	59.2
rpl36-rps8_p4	rpl36-rps8	4	CGAGACCAGGCCCTTTTATC	TCGAATTTTAGGGGGCATGG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.110972	78725	79206	1339-1339	0.500000	59.1
rpl36-rps8_p5	rpl36-rps8	5	CGAGACCAGGCCCTTTTATC	GGCATGGGGATTGTCATTCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.122760	78725	79206	1326-1326	0.500000	59.0
ccsA-ndhD_p1	ccsA-ndhD	1	TCTTCGATAGTGGCCTCCAT	TTTCGGCTGACAAGGTTGAA	20	20	50.0	45.0	57.9	58.0	3.3	0.0	0.133666	110372	110626	407-412	1.000000	81.1
ccsA-ndhD_p2	ccsA-ndhD	2	TCTTCGATAGTGGCCTCCAT	TTTTCGGCTGACAAGGTTGA	20	20	50.0	45.0	57.9	58.0	3.3	0.0	0.133666	110372	110626	408-413	1.000000	81.1
ccsA-ndhD_p3	ccsA-ndhD	3	TTCTTCGATAGTGGCCTCCA	TTTCGGCTGACAAGGTTGAA	20	20	50.0	45.0	58.1	58.0	3.3	0.0	0.184920	110372	110626	408-413	1.000000	80.7
ccsA-ndhD_p4	ccsA-ndhD	4	TTCTTCGATAGTGGCCTCCA	TTTTCGGCTGACAAGGTTGA	20	20	50.0	45.0	58.1	58.0	3.3	0.0	0.184920	110372	110626	409-414	1.000000	80.7
ccsA-ndhD_p5	ccsA-ndhD	5	CTTCGATAGTGGCCTCCATG	TTTCGGCTGACAAGGTTGAA	20	20	55.0	45.0	57.8	58.0	3.3	0.0	0.206384	110372	110626	406-411	1.000000	80.5
ycf1_p1	ycf1	1	GCTTGTGACAAAAAGGAGACA	TGCACATATAACGGCGTTCA	21	20	42.9	45.0	56.9	58.0	0.0	30.4	2.067152	121165	121284	1028-1061	1.000000	68.1
ycf1_p2	ycf1	2	TGCTTGTGACAAAAAGGAGAC	TGCACATATAACGGCGTTCA	21	20	42.9	45.0	56.9	58.0	0.0	30.4	2.067152	121165	121284	1029-1062	1.000000	68.1
ycf1_p3	ycf1	3	GCTTGTGACAAAAAGGAGACA	AACGGCGTTCAATTATCCGA	21	20	42.9	45.0	56.9	58.0	0.0	21.2	2.068549	121165	121284	1019-1052	1.000000	68.1
ycf1_p4	ycf1	4	GCTTGTGACAAAAAGGAGACA	ACGGCGTTCAATTATCCGAA	21	20	42.9	45.0	56.9	58.0	0.0	6.8	2.068549	121165	121284	1018-1051	1.000000	68.1
ycf1_p5	ycf1	5	TGCTTGTGACAAAAAGGAGAC	ACGGCGTTCAATTATCCGAA	21	20	42.9	45.0	56.9	58.0	0.0	6.8	2.068549	121165	121284	1019-1052	1.000000	68.1