Hotspot

trnfM

LSC · 13433-13504 · 72 bp

Pi 0.111

MarkerSeek score 72.60

Primer available yes

Label trnfM

Feature Metadata

label_name: trnfM
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 8
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM_p1	TTTCTAGGGCCGGGAACTAA	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.0	58.0	300-306 bp	1.0	87.9
trnfM_p2	AATTCAGCTTTCTAGGGCCG	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.0	58.0	308-314 bp	1.0	87.6
trnfM_p3	AATTCAGCTTTCTAGGGCCG	TTGTTTCAGCTCGGTGGAAA	50.0	45.0	58.0	58.0	589-595 bp	1.0	83.6
trnfM_p4	AGAAGATGCGGGTTCGATTC	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.1	58.0	547 bp	0.5	59.3
trnfM_p5	GAGAAGATGCGGGTTCGATT	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.1	58.0	548 bp	0.5	59.3