Hotspot

psaJ

LSC · 67740-67868 · 129 bp

Pi 0.0698

MarkerSeek score 69.60

Primer available yes

Label psaJ

Feature Metadata

label_name: psaJ
feature_type: gene
region: LSC
length_bp: 129
variable_sites: 9
pi: 0.0698
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ_p1	CGAACTGAACTAAGAGCGCT	TATGATTTCGTGGAGCCTGC	50.0	50.0	58.0	58.0	929-949 bp	1.0	82.3
psaJ_p2	CGAACTGAACTAAGAGCGCT	TGCTATGATTTCGTGGAGCC	50.0	50.0	58.0	58.0	932-952 bp	1.0	82.3
psaJ_p3	CGAACTGAACTAAGAGCGCT	CAAACGCCCACGAAAAGATC	50.0	50.0	58.0	58.0	1190-1224 bp	1.0	81.8
psaJ_p4	TGGTCTGGAGGAGGTCAAAT	TATGATTTCGTGGAGCCTGC	50.0	50.0	58.0	58.0	1071-1091 bp	1.0	81.7
psaJ_p5	TGGTCTGGAGGAGGTCAAAT	CAAACGCCCACGAAAAGATC	50.0	50.0	58.0	58.0	1332-1366 bp	1.0	81.4