Hotspot

atpH

LSC · 34237-34482 · 246 bp

Pi 0

MarkerSeek score 21.10

Primer available yes

Label atpH

Feature Metadata

label_name: atpH
feature_type: gene
region: LSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH_p1	AGTCATCTTTTGTGCGGGTT	AGAAGGCGAGCATATCCTCT	45.0	50.0	58.0	58.0	1018-1024 bp	1.0	81.8
atpH_p2	CATCTTTTGTGCGGGTTTCC	AGAAGGCGAGCATATCCTCT	50.0	50.0	57.9	58.0	1015-1021 bp	1.0	81.5
atpH_p3	GCGGAGTCAGGCTAGATCTA	AGAAGGCGAGCATATCCTCT	55.0	50.0	58.1	58.0	1060-1066 bp	1.0	81.1
atpH_p4	AGTCATCTTTTGTGCGGGTT	TGATCACTTACTTCGCTCGC	45.0	50.0	58.0	58.1	1308-1313 bp	1.0	80.8
atpH_p5	AGTCATCTTTTGTGCGGGTT	ATCAGCCTGGGGTATTGTCT	45.0	50.0	58.0	58.1	981 bp	0.5	59.0