Hotspot

ndhF-rpl32

SSC · 108531-109359 · 829 bp

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Pi 0.0145
MarkerSeek score 66.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
829
variable_sites
12
pi
0.0145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAGGAAGTGGGAGAAAAGG TCCAAAGATTTTTACGAATACGCT 50.0 33.3 57.0 57.3 942-943 bp 1.0 41.1
ndhF-rpl32_p2 CAGGAAGTGGGAGAAAAGGT TCCAAAGATTTTTACGAATACGCT 50.0 33.3 57.0 57.3 941-942 bp 1.0 41.1
ndhF-rpl32_p3 AACAGGAAGTGGGAGAAAAGG TCCAAAGATTTTTACGAATACGCT 47.6 33.3 57.8 57.3 943-944 bp 1.0 41.1
ndhF-rpl32_p4 ACAGGAAGTGGGAGAAAAGG TCCAAAGATTTTTACGAATACGC 50.0 34.8 57.0 55.9 942-943 bp 1.0 41.1
ndhF-rpl32_p5 CAGGAAGTGGGAGAAAAGGT TCCAAAGATTTTTACGAATACGC 50.0 34.8 57.0 55.9 941-942 bp 1.0 41.1