Hotspot

rps3-rpl22

LSC · 82590-82646 · 57 bp

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Pi 0.0182
MarkerSeek score 64.50
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
57
variable_sites
1
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 GTGCGAACCAAAAGGAATGG AAATTCAGACCTCGAGCTCG 50.0 50.0 57.9 57.7 224-226 bp 1.0 78.3
rps3-rpl22_p2 ATTTTTCGGTTGTGCGAACC AAATTCAGACCTCGAGCTCG 45.0 50.0 57.9 57.7 235-237 bp 1.0 77.7
rps3-rpl22_p3 GTTGTGCGAACCAAAAGGAA AAATTCAGACCTCGAGCTCG 45.0 50.0 57.4 57.7 227-229 bp 1.0 74.2
rps3-rpl22_p4 GTGCGAACCAAAAGGAATGG TGAGTAGGAGCGCTATTGTG 50.0 50.0 57.9 57.1 247-249 bp 1.0 73.5
rps3-rpl22_p5 GTGCGAACCAAAAGGAATGG GTGAGTAGGAGCGCTATTGT 50.0 50.0 57.9 57.1 248-250 bp 1.0 73.5