Hotspot

psaJ-rpl33

LSC · 67869-68193 · 325 bp

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Pi 0.0502
MarkerSeek score 68.50
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
325
variable_sites
16
pi
0.0502
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 CGAACTGAACTAAGAGCGCT CAAACGCCCACGAAAAGATC 50.0 50.0 58.0 58.0 1190-1224 bp 1.0 81.8
psaJ-rpl33_p2 GCGTTCTACCGAACTGAACT CAAACGCCCACGAAAAGATC 50.0 50.0 57.9 58.0 1199-1233 bp 1.0 81.5
psaJ-rpl33_p3 CGAACTGAACTAAGAGCGCT TAATCGATTCGATCCCCCGA 50.0 50.0 58.0 58.1 1216-1250 bp 1.0 81.4
psaJ-rpl33_p4 CGAACTGAACTAAGAGCGCT GAATTCCCCGGAGAAGGAAC 50.0 55.0 58.0 58.0 1613 bp 0.5 59.6
psaJ-rpl33_p5 GCGTTCTACCGAACTGAACT GAATTCCCCGGAGAAGGAAC 50.0 55.0 57.9 58.0 1622 bp 0.5 59.2