Hotspot

psbF-psbE

LSC · 65111-65120 · 10 bp

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Pi 0.5
MarkerSeek score 72.50
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
10
variable_sites
5
pi
0.5
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 AGTATCGGCCATTTCCCCTA TGCGGGTTGGTTATTTGTCA 50.0 45.0 57.9 58.0 563 bp 1.0 81.0
psbF-psbE_p2 TATCGGCCATTTCCCCTACT TGCGGGTTGGTTATTTGTCA 50.0 45.0 57.9 58.0 561 bp 1.0 81.0
psbF-psbE_p3 TAGTATCGGCCATTTCCCCT TGCGGGTTGGTTATTTGTCA 50.0 45.0 57.9 58.0 564 bp 1.0 80.9
psbF-psbE_p4 TATCGGCCATTTCCCCTACT CTGTTTCGGGACCAAGTGAA 50.0 50.0 57.9 58.0 858 bp 1.0 80.1
psbF-psbE_p5 TATCGGCCATTTCCCCTACT TTCTGTTTCGGGACCAAGTG 50.0 50.0 57.9 58.0 860 bp 1.0 80.1