Hotspot

trnG-trnT

LSC · 14356-15708 · 1353 bp

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Pi 0.0363
MarkerSeek score 67.40
Primer available yes
Label trnG-trnT

Feature Metadata

label_name
trnG-trnT
feature_type
igs
region
LSC
length_bp
1353
variable_sites
49
pi
0.0363
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-trnT_p1 ACGAATCACACTTTTACCACT TTGAACCGATGACTTACGCC 38.1 50.0 55.5 58.3 1444-1450 bp 1.0 52.3
trnG-trnT_p2 AACGAATCACACTTTTACCACT TTGAACCGATGACTTACGCC 36.4 50.0 56.2 58.3 1445-1451 bp 1.0 50.3
trnG-trnT_p3 ACGAATCACACTTTTACCACT CGATGACTTACGCCTTACCA 38.1 50.0 55.5 57.4 1438-1444 bp 1.0 50.0
trnG-trnT_p4 ACGAATCACACTTTTACCACT TGACTTACGCCTTACCATGG 38.1 50.0 55.5 57.3 1435-1441 bp 1.0 49.1
trnG-trnT_p5 ACGAATCACACTTTTACCACT GACTTACGCCTTACCATGGC 38.1 55.0 55.5 58.7 1434-1440 bp 1.0 48.9