Hotspot

trnfM-trnG

LSC · 13505-13599 · 95 bp

Back to Pharus

Pi 0.112
MarkerSeek score 78.00
Primer available yes
Label trnfM-trnG

Feature Metadata

label_name
trnfM-trnG
feature_type
igs
region
LSC
length_bp
95
variable_sites
10
pi
0.112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-trnG_p1 TTTCTAGGGCCGGGAACTAA AGCCTTCCAAGCTAACGATG 50.0 50.0 58.0 58.0 300-306 bp 1.0 87.9
trnfM-trnG_p2 AATTCAGCTTTCTAGGGCCG AGCCTTCCAAGCTAACGATG 50.0 50.0 58.0 58.0 308-314 bp 1.0 87.6
trnfM-trnG_p3 AATTCAGCTTTCTAGGGCCG TTGTTTCAGCTCGGTGGAAA 50.0 45.0 58.0 58.0 589-595 bp 1.0 83.6
trnfM-trnG_p4 AGAAGATGCGGGTTCGATTC AGCCTTCCAAGCTAACGATG 50.0 50.0 58.1 58.0 547 bp 0.5 59.3
trnfM-trnG_p5 GAGAAGATGCGGGTTCGATT AGCCTTCCAAGCTAACGATG 50.0 50.0 58.1 58.0 548 bp 0.5 59.3