Hotspot

psbZ-trnfM

LSC · 12756-13432 · 677 bp

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Pi 0.0859
MarkerSeek score 80.00
Primer available yes
Label psbZ-trnfM

Feature Metadata

label_name
psbZ-trnfM
feature_type
igs
region
LSC
length_bp
677
variable_sites
53
pi
0.0859
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM_p1 CAACCACTCAGCCATCTCTC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.0 58.0 1436-1446 bp 1.0 87.9
psbZ-trnfM_p2 CAACCACTCAGCCATCTCTC CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.0 58.1 1439-1449 bp 1.0 87.7
psbZ-trnfM_p3 GAGCTATCAACCACTCAGCC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.1 58.0 1443-1453 bp 1.0 87.6
psbZ-trnfM_p4 GGAGCTATCAACCACTCAGC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.1 58.0 1444-1454 bp 1.0 87.6
psbZ-trnfM_p5 CAACCACTCAGCCATCTCTC TTGTTTCAGCTCGGTGGAAA 55.0 45.0 58.0 58.0 1717-1727 bp 1.0 86.4