Hotspot

ycf1

SSC · 126621-131678 · 5058 bp

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Pi 0.0663

MarkerSeek score 77.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5058
variable_sites: 1190
pi: 0.0663
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCAACGTTTCGGAATCGAT	CCAAACGACAAGAAAAAGGGA	45.0	42.9	57.9	56.9	1264-1414 bp	0.846154	68.6
ycf1_p2	ACCAACGTTTCGGAATCGAT	TCCAAACGACAAGAAAAAGGG	45.0	42.9	57.9	56.9	1265-1415 bp	0.846154	68.6
ycf1_p3	AACCAACGTTTCGGAATCGA	CCAAACGACAAGAAAAAGGGA	45.0	42.9	58.1	56.9	1265-1415 bp	0.846154	68.3
ycf1_p4	CCCGTATTTTGCGTGGTTTC	CCAAACGACAAGAAAAAGGGA	50.0	42.9	58.0	56.9	995-1064 bp	0.307692	43.7
ycf1_p5	CCCGTATTTTGCGTGGTTTC	TCCAAACGACAAGAAAAAGGG	50.0	42.9	58.0	56.9	996-1065 bp	0.307692	43.7